M.Sc. (1973) : Technical University of Budapest, Hungary
Ph.D. (1979) : Technical University of Budapest, Hungary
Visiting Associate Professor (1982-1984) : Emory University
Our studies are centered on analytical chemistry applications with special emphasis on bioanalytical analyses. Two major analytical techniques are used: fluorescence spectroscopy and capillary electrophoresis (CE). Our fluorescence spectroscopy research efforts are focused on the longer wavelength region of the electromagnetic spectrum, the near-infrared (NIR) region. The NIR region is advantageous due to the inherent low interference and the high molar absorptivities of the NIR absorbing chromophores. When NIR spectroscopy is coupled with the use of semiconductor lasers and detectors, an especially useful technique emerges. Semiconductor lasers have all the properties of other types of lasers with the added benefits of compactness, lower power consumption and low cost.
We are currently studying several bioanalytical applications. These include, but are not limited to, NIR DNA sequencing; NIR fluorescence immunoassays, including fiber optic and conventional applications; DNA probe techniques; pH, metal ion and hydrophobicity determinations. Additionally, new environmentally important NIR analytical techniques are studied in our research group, including separation and bioremediation applications. Another component of our studies is the development of new biosensors using fiber optic techniques. Our CE research is mainly focused on new high sensitivity detection methods, bioseparations and NIR labeling techniques.
The latest area of our research is development of new spectroscopic method for forensic applications. Our research group developed a new latent blood detection method using new fluorescin chemistry. In addition to latent blood detection we also develop new analytical techniques for latent fingerprint detection and determination of other chemical residues, e.g., pepper spray residues, gun shot residues and explosive residues. The most promising part of our research is the combination of forensic analytical chemistry with NIR spectroscopy.
- Testino, Samuel A.; Patonay, Gabor, High-throughput inhibition screening of major human cytochrome P450 enzymes using an in vitro cocktail and liquid chromatography-tandem mass spectrometry, Journal of Pharmaceutical and Biomedical Analysis (2003), 30(5), 1459-1467.
- L. Strekowski, Mason, C.L , Lee, H,. R. Gupta, J. Sowell, and Gabor Patonay, Syntesis of Water Soluble Near-Infrared Cyanine Dyes Functionalized with [(Succinimido)oxy]carbonyl Group, J. Hetercyc. Chem, (2003) 40, 913-916,.
- Ellis,A.L., Mason,J.C., Lee,H.W., Strekowski,L., Patonay,G., Choi,H., and Yang,J.J. (2002). Design, synthesis, and characterization of a calcium-sensitive near infrared dye. Talanta, 56, 1099-1107.
- Tarazi,L., Choi,H., Mason,J.C., Sowell,J., Strekowski,L., and Patonay,G. (2002) Characterization of a novel crown ether-bearing near-infrared heptamethine cyanine dye. A study of fluorescence quenching by lithium. Microchemical Journal, 72, 55-62.
- Tarazi,L., Narayanan,N., Sowell,J., Patonay,G., and Strekowski,L. (2002). Investigation of the spectral properties of a squarylium near-infrared dye and its complexation with Fe(III) and Co(II) ions. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 58, 257-264.
- Strekowski,L., Gorecki,T., Mason,J.C., Lee,H., and Patonay,G. (2001). New heptamethine cyanine reagents for labeling of biomolecules with a near-infrared chromophore. Heterocyclic Communications, 7, 117-122.
- Sowell,J., Agnew-Heard,K.A., Mason,J.C., Mama,C., Strekowski,L., and Patonay,G. (2001). Use of non-covalent labeling in illustrating ligand binding to human serum albumin via affinity capillary electrophoresis with near-infrared laser induced fluorescence detection. Journal of Chromatography B, 755, 91-99.
- Strekowski,L., Mason,J.C., Britton,J.E., Lee,H., Van Aken,K., and Patonay,G. (2000). The addition reaction of hydroxide or ethoxide ion with benzindolium heptamethine cyanine dyes. Dyes and Pigments,46 163-168.